Development of DNA markers linked to double-flower and hortensia traits in Hydrangea macrophylla (Thunb.) Ser.

Takamitsu Waki, Masaharu Kodama, Midori Akutsu, Kiyoshi Namai, Masayuki Iigo, Takeshi Kurokura, Toshiya Yamamoto, Kenji Nashima, Masayoshi Nakayama, Masafumi Yagi

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18 Citations (Scopus)


Double flower and hortensia (mophead) hydrangea (Hydrangea macrophylla (Thunb.) Ser.) traits are recessively inherited. Cross breeding of these traits in hydrangea is difficult because it takes about two years from crossing to flowering. In this study, we aimed to obtain DNA linkage markers that would allow accelerated selection of these traits. We used next-generation sequencing to comprehensively collect DNA sequences from the ‘Kirakiraboshi’ with a double flower and lacecap inflorescence and the ‘Frau Yoshimi’ with a single flower and hortensia inflorescence, and designed simple sequence repeat (SSR) primer pairs for map construction. We screened 768 SSR primer pairs in 93 F2 progeny derived from ‘Kirakiraboshi’ and ‘Frau Yoshimi’. We identified 147 loci, which were expanded to 18 linkage groups with a total map length of 980 cM. Linkage analysis identified that both the double flower trait from ‘Kirakiraboshi’ (dKira) and the hortensia trait from ‘Frau Yoshimi’ (hFrau) were located on linkage group KF_4. Detailed linkage analysis using 351 F2 progeny revealed a 34.8 cM map length between the two loci and identified two tightly linked SSR markers, STAB045 for dKira and HS071 for hFrau. Genetic analysis suggested that double flower and hortensia traits are each controlled by a single recessive gene. Together, the linkage map, SSR markers, and genetic information obtained in this study will be useful for future hydrangea breeding.

Original languageEnglish
Pages (from-to)264-273
Number of pages10
JournalHorticulture Journal
Issue number2
Publication statusPublished - 2018


  • Flower type
  • Inflorescence
  • Linkage map
  • Mophead
  • Simple sequence repeat (SSR)


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