Detection of Ubiquitinated Dermcidin in Gingival Crevicular Fluid in Periodontal Disease

Sachio Tsuchida, Mamoru Satoh, Masaki Takiwaki, Michiru Wakabayashi, Takayuki Ishige, Minako Beppu, Motoi Nishimura, Kazuyuki Matsushita, Fumio Nomura

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Periodontal disease is an inflammatory disease caused by gram-negative bacteria, such as Porphyromonas gingivalis and Actinobacllus actinomycetemcomitans. Antimicrobial peptides kill organisms, such as gram-negative and gram-positive bacteria, mycobacteria, enveloped viruses, and fungi. We previously identified the antimicrobial peptide dermcidin (DCD) in the gingival crevicular fluid (GCF) using proteomic analyses. Moreover, western blot analysis revealed that the molecular weights of GCF protein bands considerably varied (approximately 27 kDa). We attempted to explore the considerable variation in the molecular weights of protein bands using on-membrane digestion and liquid chromatography–tandem mass spectrometry (LC–MS/MS) analyses. We examined ubiquitin among the DCD-interacting proteins. In immunoprecipitation experiments, ubiquitinated DCD was detected by western blotting and by immunoprecipitation with an antibody against DCD and mono- or poly-ubiquitinated proteins. These analyses indicated the possible involvement of the ubiquitination reaction. Ubiquitinated DCD may protect against periodontal bacterial pathogen invasion in GCF.

Original languageEnglish
Pages (from-to)249-253
Number of pages5
JournalInternational Journal of Peptide Research and Therapeutics
Issue number2
Publication statusPublished - 1 Jun 2016
Externally publishedYes


  • Gingival crevicular fluid
  • Periodontal disease
  • Proteomics


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