TY - JOUR
T1 - Design and fabrication of customized brain slice matrices using CAD and 3D printing technology
AU - Yamazaki, Yosuke
AU - Yuguchi, Maki
AU - Honjo, Bin
AU - Isokawa, Keitaro
N1 - Publisher Copyright:
© 2025 Yamazaki et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2025/1
Y1 - 2025/1
N2 - This study presents a novel method for creating customized brain slice matrices using Computer-Aided Design (CAD) and 3D printing technology. Brain Slice Matrices are essential jigs for the reproducible preparation of brain tissue sections in neuroscience research. Our approach leverages the advantages of 3D printing, including design flexibility, cost-effectiveness, and rapid prototyping, to produce custom-made brain matrices based on specific morphometric measurements. The developed protocol is user-friendly and incorporates features such as embossed identifying markers and support structures for challenging thin regions, thereby enhancing its practical utility. Our method demonstrates rapid and cost-effective fabrication of custom brain matrices, significantly reducing both material expenses and production time compared to traditional manufacturing techniques and ready-made products. This work contributes to the growing application of 3D printing in biomedical research, offering a valuable tool for neuroscientists requiring precise and consistent brain tissue sectioning.
AB - This study presents a novel method for creating customized brain slice matrices using Computer-Aided Design (CAD) and 3D printing technology. Brain Slice Matrices are essential jigs for the reproducible preparation of brain tissue sections in neuroscience research. Our approach leverages the advantages of 3D printing, including design flexibility, cost-effectiveness, and rapid prototyping, to produce custom-made brain matrices based on specific morphometric measurements. The developed protocol is user-friendly and incorporates features such as embossed identifying markers and support structures for challenging thin regions, thereby enhancing its practical utility. Our method demonstrates rapid and cost-effective fabrication of custom brain matrices, significantly reducing both material expenses and production time compared to traditional manufacturing techniques and ready-made products. This work contributes to the growing application of 3D printing in biomedical research, offering a valuable tool for neuroscientists requiring precise and consistent brain tissue sectioning.
UR - http://www.scopus.com/inward/record.url?scp=85215425287&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0317616
DO - 10.1371/journal.pone.0317616
M3 - Article
C2 - 39823510
AN - SCOPUS:85215425287
SN - 1932-6203
VL - 20
JO - PLoS ONE
JF - PLoS ONE
IS - 1
M1 - e0317616
ER -