TY - JOUR
T1 - Characterization of feline TRIM genes
T2 - Molecular cloning, expression in tissues, and response to type I interferon
AU - Koba, Ryota
AU - Kokaji, Chika
AU - Fujisaki, Gentoku
AU - Oguma, Keisuke
AU - Sentsui, Hiroshi
PY - 2013
Y1 - 2013
N2 - Members of the tripartite motif (TRIM) protein family in mammals are responsible for various cellular processes. Previous studies have revealed that several TRIM proteins were induced by interferons (IFN) and that these proteins were involved in innate immune response against retroviral infection. Although retroviral infection is prevalent in domestic cats, the expression profiles and roles of feline TRIM genes against these viral infections are not well understood. In the present study, we examined tissue expression and IFN inducibility of nine feline TRIM genes. In addition, the complete coding sequences of six cloned TRIM genes were determined, and their structures were analyzed. Nine TRIM genes were expressed in feline tissues and five were up-regulated by type I IFN. The predicted amino acid sequence of six feline TRIM proteins showed high sequence similarities to other mammalian TRIM proteins, and suggest that feline TRIM genes are potentially involved in antiviral reactivity in IFN-mediated immune response.
AB - Members of the tripartite motif (TRIM) protein family in mammals are responsible for various cellular processes. Previous studies have revealed that several TRIM proteins were induced by interferons (IFN) and that these proteins were involved in innate immune response against retroviral infection. Although retroviral infection is prevalent in domestic cats, the expression profiles and roles of feline TRIM genes against these viral infections are not well understood. In the present study, we examined tissue expression and IFN inducibility of nine feline TRIM genes. In addition, the complete coding sequences of six cloned TRIM genes were determined, and their structures were analyzed. Nine TRIM genes were expressed in feline tissues and five were up-regulated by type I IFN. The predicted amino acid sequence of six feline TRIM proteins showed high sequence similarities to other mammalian TRIM proteins, and suggest that feline TRIM genes are potentially involved in antiviral reactivity in IFN-mediated immune response.
KW - Cat
KW - Innate immunity
KW - Interferon
KW - Tripartite motif (TRIM)
UR - http://www.scopus.com/inward/record.url?scp=84884729428&partnerID=8YFLogxK
U2 - 10.1016/j.vetimm.2013.02.009
DO - 10.1016/j.vetimm.2013.02.009
M3 - Article
C2 - 23497841
AN - SCOPUS:84884729428
SN - 0165-2427
VL - 153
SP - 91
EP - 98
JO - Veterinary Immunology and Immunopathology
JF - Veterinary Immunology and Immunopathology
IS - 1-2
ER -