A pilot study of microRNA assessment as a means to identify novel biomarkers of spontaneous osteoarthritis in dogs

Atsushi Yamazaki, Yuma Tomo, Hinano Eto, Koji Tanegashima, Kazuya Edamura

Research output: Contribution to journalArticlepeer-review

Abstract

MicroRNAs (miRNAs) are important regulators of intercellular signaling and are promising biomarkers in osteoarthritis (OA). In this study, comprehensive analysis was performed to identify miRNAs involved in the pathogenesis of spontaneous OA in dogs. Dogs diagnosed with OA based on radiography and arthroscopy of the stifle joint were included in the OA group. Dogs without any evidence of orthopedic disease were included in the unaffected group. To investigate miRNA expression levels, RNA sequencing analysis (RNA-seq) was performed in synovial tissue (OA group: n = 3, Unaffected group: n = 3) and RT-qPCR was performed in synovial tissue, synovial fluid and serum (OA group: n = 17, Unaffected group: n = 6), and compared between the two groups. The RNA-seq results showed that 57 miRNAs were significantly upregulated and 42 were significantly downregulated in the OA group. Specifically, miR-542 and miR-543 expression levels in the synovial tissue, synovial fluid, and serum were consistently higher in the OA group than in the unaffected group, suggesting that these miRNAs may be used as biomarkers for detecting canine OA. This is the first report to comprehensively analyze the expression patterns of miRNAs in the synovial tissue of dogs with spontaneous OA.

Original languageEnglish
Article number18152
JournalScientific Reports
Volume12
Issue number1
DOIs
Publication statusPublished - Dec 2022

Fingerprint

Dive into the research topics of 'A pilot study of microRNA assessment as a means to identify novel biomarkers of spontaneous osteoarthritis in dogs'. Together they form a unique fingerprint.

Cite this